Application

Research Sub CategoryFragment Specific Secondary Antibodies

ELISA: 1:4,000-1:8,000.

Optimal working dilutions must be determined by the end user.

Research CategorySecondary & Control Antibodies

This Goat anti-Mouse IgM+IgG+IgA Antibody, F(ab′)2, HRP conjugate, Species Adsorbed is validated for use in ELISA for the detection of Mouse IgM+IgG+IgA.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgG is the most abundant immunoglobulin and comprises two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Immunogen

Prepared from pepsin digest of mouse Ig (H+L).

Epitope: Mouse IgM, IgG, IgA

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Gel filtration chromatography

Purified by gel filtration chromatography of pepsin digested antibody. Liquid in 50% glycerol/50% PBS, pH 7.4.

Specificity

Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.

The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Storage and Stability

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. For long term storage, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.